By Sherwin B. Nuland
Original yr of publication: 2003; 2004 - pb
"Riveting" (Houston Chronicle), "captivating" (Discover), and "compulsively readable" (San Francisco Chronicle)
Surgeon, pupil, best-selling writer, Sherwin B. Nuland tells the unusual tale of Ignác Semmelweis with urgency and the perception received from his personal reviews and scientific adventure. Ignác Semmelweis is remembered for the now-commonplace thought that medical professionals needs to wash their palms earlier than reading sufferers. In mid-nineteenth-century Vienna, in spite of the fact that, this used to be a subversive proposal. With deaths from childbed fever exploding, Semmelweis came across that medical professionals themselves have been spreading the illness. whereas his uncomplicated reforms labored immediately—childbed fever in Vienna all yet disappeared—they introduced down upon Semmelweis the wrath of the institution, and ended in his tragic end.
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Extra resources for The Doctors' Plague: Germs, Childbed Fever, and the Strange Story of Ignác Semmelweis
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The corresponding values of diffusion parameters were found to be the highest in case of uninfected cells. This observation points out the potential of lateral dynamics as an indicator of progress of infection. In another study, HIV-1 fusion and entry into target cells have been shown to be dependent on the lateral mobility of CD4 receptors (which serve as one of the receptors for viral entry) in host cell membranes (Rawat et al. 2008). Conclusion and Future Perspectives Although we have discussed only representative examples of the application of FRAP in membrane and receptor biology, it is clear that this approach is capable of providing a variety of information depending on experimental design and question asked.
1 illustrates the basic principles of FRAP measurements. In this review, we will provide an overview of the range of research problems that could be addressed in membrane and receptor biology using FRAP, taking representative examples mostly from work carried out in our laboratory. This review is by no means an exhaustive review of FRAP methodology and its application in membrane biology. 3 FRAP in Membrane Biology 29 Fig. 1 Basic design of FRAP measurements. Fi represents the initial total fluorescence intensity in the region of interest (ROI) prior to photobleaching.
Prog Neurobiol 81:133–178 Nygaard R, Zou Y, Dror RO, Mildorf TJ, Arlow DH, Manglik A, Pan AC, Liu CW, Fung JJ, Bokoch MP, Thian FS, Kobilka TS, Shaw DE, Mueller L, Prosser RS, Kobilka BK (2013) The dynamic process of β2-adrenergic receptor activation. Cell 152:532–542 Parker PD, Tilley L, Klonis N (2004) Plasmodium falciparum induces reorganization of host membrane proteins during intraerythrocytic growth. Blood 103:2404–2406 Peters R (1988) Lateral mobility of proteins and lipids in the red cell membrane and the activation of adenylate cyclase by β-adrenergic receptors.