By Uwe Theo Bornscheuer, DGF
Enzymatic equipment of lipid amendment, rather of fat and oils, have built swiftly because the Eighties. In parallel to the quick development in examine a variety of functions have emerged, e.g. within the nutrition industry.The ebook is written by way of top specialists within the box and displays the cutting-edge of enzymatic lipid amendment. It presents the reader with directions tips to choose appropriate enzymes and the way to use them efficiently.Applications of lipases and phospholipases, lipoxygenases and P450-monooxygenases and using whole-cell structures in lipid amendment are defined. Cloning, expression and mutagenesis in addition to makes an attempt to appreciate the molecular foundation of specificity and stereoselectivity are defined. additionally engineering elements and the alternative of solvent platforms are addressed.
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Additional resources for Enzymes in Lipid Modification
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A. 90 80 89 72 96 22 25 705 1 26 31 MG 60 55 FFA 0 12 5 8 2 75 5 4 134 DG Product composition2 (% w/w) Maximum yield on molar basis. Product composition on weight basis in the lipid fraction, as recalculated from experimental data reported in literature. Lipase immobilised on Celite. Sum of diglycerides and triglycerides. Sum of free fatty acids and ethylesters. 2 – 5% (w/w) water in glycerol. Esterification 96 90 86 76 55 – 25 25 – 10 Pseudomonas cepacia Chromobacterium viscosum 75 – 92 67 80 95 60 – 81 Yield1 (%) 50 40 60 – 40 40 35 35 35 T (8C) Pseudomonas fluorescens Substrate: fatty acids/glycerol Directed glycerolysis Substrate: triglycerides Hydrolysis Porcine pancreatic lipase3 R.
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