By Abhijit Chakrabarti, Avadhesha Surolia
Consists of serious reports and unique examine papers from the 2014 overseas Symposium at the "Biochemical function of Eukaryotic mobilephone floor Macromolecules". themes coated contain: · neurochemical and biochemical research of mobile floor glycoconjugates · membrane skeletal association · GPCRs and different receptors · biophysical methods to review membrane interactions · glycoconjugate metabolism · dysregulation · molecular mechanisms keen on cell-cell and cell-matrix interplay · glycans in infectious and neurological ailments · melanoma and glycosyltransferases as drug targets.
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The corresponding values of diffusion parameters were found to be the highest in case of uninfected cells. This observation points out the potential of lateral dynamics as an indicator of progress of infection. In another study, HIV-1 fusion and entry into target cells have been shown to be dependent on the lateral mobility of CD4 receptors (which serve as one of the receptors for viral entry) in host cell membranes (Rawat et al. 2008). Conclusion and Future Perspectives Although we have discussed only representative examples of the application of FRAP in membrane and receptor biology, it is clear that this approach is capable of providing a variety of information depending on experimental design and question asked.
1 illustrates the basic principles of FRAP measurements. In this review, we will provide an overview of the range of research problems that could be addressed in membrane and receptor biology using FRAP, taking representative examples mostly from work carried out in our laboratory. This review is by no means an exhaustive review of FRAP methodology and its application in membrane biology. 3 FRAP in Membrane Biology 29 Fig. 1 Basic design of FRAP measurements. Fi represents the initial total fluorescence intensity in the region of interest (ROI) prior to photobleaching.
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