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By G. Gubitz, M. Schmid
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A 792, 401–409. 11. , and Mosbach, K. (1989) Molecular imprinting of amino acid derivatives at low temperature (°C) using photolytic homolysis of azobisnitriles. Anal. Biochem. 177, 144–149. 12. , Andersson, L. , and Nilsson, S. (2001) Rapid electrochromatographic enantiomer separations on short molecularly imprinted polymer monoliths. Anal. Chim. Acta 435, 43–47. 13. Schweitz, L. (2002) Molecularly imprinted polymer coatings for open-tubular capillary electrochromatography prepared by surface initiation.
Methanol and methanol/acetic acid (9:1, v/v) (MIP washing solutions), syringe and PVC tubing (see step 2). 4. Preparation of OT-MIP 1. Dichloromethane (polymerization solvent), MAA (functional monomer), TRIM (crosslinking monomer), free base S-propranolol (template molecule). Glass vial (1 mL) with cover. Be aware of that all monomers are toxic and potential skin sensitizers. CEC Using Molecularly Imprinted Polymers 419 2. Syringe (outer diameter of the needle 330 µm), PVC tubing (inner diameter 127 µm), soft plastic rubber.
418 Spégel et al. 2. Preparation of Capillaries for OT-MIP 1. , from Composite Metal Services; cat. no. 1 M NaOH, 1 M HCl, distilled water, acetone, nitrogen gas, and toluene (HPLC-grade) dried over molecular sieve. Syringe (outer diameter of the needle 330 µm), PVC tubing (inner diameter 127 µm). 2. Dry toluene (HPLC-grade)/3-aminopropyltriethoxysilane (85:15, v/v) (amino derivatization solution), syringe and PVC tubing (see step 1) and soft plastic rubber. The silanization solution should be prepared fresh as required.