By Bernard Moret, Henry D. Shapiro

Ebook by means of Moret, Bernard, Shapiro, Henry D.

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The periodic acid–Schiff (PAS) reaction depends on the liberation of carbohydrate radicals, which are oxidized to aldehydes and then reacted with Schiff reagent. Positivity in hemopoietic cells usually denotes glycogen, which is prominent in malignant erythroid precursors (blush and block positive). Granules can also be seen in some lymphoid and some myeloid leukemias. 6% of ALL cases have 5% or greater SBB positivity). Naphthol AS-D chloroacetate esterase (NASD), found in granulocytic precursors, cleaves the naphthyl group, which combines with a diazonium salt to give an azo dye.

12 Section 1: Basic science (continued) Group Selectins (leccams) Intercellular adhesion molecules Addressins Molecular characteristics Terminal lectinlike domain, thought to bind sugar residues Members of immunoglobulin gene superfamily Single chain Example (Function) Ligand Function E-selectin (ELAM-1) L-selectin (Mel-14) P-selectin (GMP140, CD62) ICAM-1 ICAM-2 VCAM Rolling (superficial attachment) Sialyl Lewis-X Sulfated glycoprotein LFA-1 LFA-2 VLA-4 Extravasation Endothelial receptors for lymphocyte homing Adhesion molecules Adhesion molecules are important in promoting interactions between leukocytes and for attachment of leukocytes to endothelium.

3. Indication Disease-free survival (DFS) (%) AML in CR1 AML in untreated first relapse or CR2 APL in CR2 40–50 20–30 40–70 HLA-Identical Unrelated Donor Transplant 1. 2. , 2008). Pretransplant workup 1. Evaluation of peripheral blood and bone marrow: Morphology, cytochemistry, immune phenotype, cytogenetics (and molecular testing as appropriate) 2. See the general pretransplant workup (page 240). Monitoring posttransplant 1. Bone marrow aspirate, with cytogenetic evaluation (and molecular evaluation, if appropriate) at 1, 3, 12, and 24 months.

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